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caption a7 plasmid description references pmetαb vector  (Thermo Fisher)


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    Thermo Fisher caption a7 plasmid description references pmetαb vector
    Caption A7 Plasmid Description References Pmetαb Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caption+a7+vector/pmc07058725-211-18-29
    Average 86 stars, based on 1 article reviews
    caption a7 plasmid description references pmetαb vector - by Bioz Stars, 2026-11
    86/100 stars

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    Article Title: Evidence for a Gender-Specific Protective Role of Innate Immune Receptors in a Model of Perinatal Brain Injury
    Article Snippet: All images were captured using a Nikon Eclipse 80i microscope equipped with a digital camera (QImaging) and processed to enhance contrast and brightness using Adobe Photoshop CS6 (Version 13.0; Adobe Systems). table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Mouse plasmid Vector Length (bp) Enzymes used for the sense probe Enzymes used for the antisense probe Source CTGF pCMV-Sport6 2334 XhoI/Sp6 BamHI/T7 Thermo Scientific Fractalkine pCRII-TOPO 1095 BamHI/T7 XhoI/Sp6 Cloned by PCR TLR2 PCR-blunt II 2248 Spe/T7 EcoRV/Sp6 Cloned by PCR TLR3 pCRII-TOPO 1645 XhoI/Sp6 BamHI/T7 Cloned by PCR TLR6 pZero-2 2304 XhoI/Sp6 BamHI/T7 Dr. C.B.

    Article Title: Heterologous secretory expression of β-glucosidase from Thermoascus aurantiacus in industrial Saccharomyces cerevisiae strains
    Article Snippet: The С-terminal 1xflag-tagged tabgl1 gene ( {"type":"entrez-nucleotide","attrs":{"text":"DQ114397.1","term_id":"74054463","term_text":"DQ114397.1"}} DQ114397.1 , GenBank) was synthesized artificially (GeneCust Europe, Dudelange – Luxembourg) for expression in S . cerevisiae but with the native secretion signal sequences omitted.

    Article Title: A comparison and optimization of yeast two-hybrid systems
    Article Snippet: These vectors are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Gal4-Fusion Selection Vector Promoter N / C AD/DBD yeast bacterial ori Source pDEST22 fl-ADH1 N AD Trp1 Ampicillin CEN Invitrogen pDEST32 * fl-ADH1 N DBD Leu2 Gentamicin CEN Invitrogen pGBKT7g t-ADH1 N DBD Trp1 Kanamycin 2μ [ 15 ] pGADT7g fl-ADH1 N AD Leu2 Ampicillin 2μ [ 15 ] pGBGT7g t-ADH1 N DBD Trp1 Gentamicin 2μ pGADCg fl-ADH1 C AD Leu Ampicillin 2μ [ 9 ] pGBKCg t-ADH1 C DBD Trp Kanamycin 2μ [ 9 ] Open in a separate window * also encodes CYH2; fl-, t-ADH1 = full length and truncated ADH1 promoters.

    Polymerase Chain Reaction:

    Article Title: Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus
    Article Snippet: .. Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2 caption a7 Double digestion of recombinant vector by Bam HI and Hind III restriction enzymes that lead to excision of core-E1-E2 fusion gene. .. Lane 1: digested vector and core-E1-E2 fusion fragment; Lane 2: recombinant vector linearized by Bam HI; Lane M: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 3 caption a7 Detection of core-E1-E2 mRNA in transfected and non-transfected Huh- 7.5 cells by RT-PCR analysis.

    Marker:

    Article Title: Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus
    Article Snippet: .. Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2 caption a7 Double digestion of recombinant vector by Bam HI and Hind III restriction enzymes that lead to excision of core-E1-E2 fusion gene. .. Lane 1: digested vector and core-E1-E2 fusion fragment; Lane 2: recombinant vector linearized by Bam HI; Lane M: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 3 caption a7 Detection of core-E1-E2 mRNA in transfected and non-transfected Huh- 7.5 cells by RT-PCR analysis.

    Recombinant:

    Article Title: Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus
    Article Snippet: .. Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2 caption a7 Double digestion of recombinant vector by Bam HI and Hind III restriction enzymes that lead to excision of core-E1-E2 fusion gene. .. Lane 1: digested vector and core-E1-E2 fusion fragment; Lane 2: recombinant vector linearized by Bam HI; Lane M: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 3 caption a7 Detection of core-E1-E2 mRNA in transfected and non-transfected Huh- 7.5 cells by RT-PCR analysis.

    Plasmid Preparation:

    Article Title: Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus
    Article Snippet: .. Lane 1, 2: a 224 bp PCR product; Lane 3: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 2 caption a7 Double digestion of recombinant vector by Bam HI and Hind III restriction enzymes that lead to excision of core-E1-E2 fusion gene. .. Lane 1: digested vector and core-E1-E2 fusion fragment; Lane 2: recombinant vector linearized by Bam HI; Lane M: 1kb DNA size marker (Fermentas, Germany fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 3 caption a7 Detection of core-E1-E2 mRNA in transfected and non-transfected Huh- 7.5 cells by RT-PCR analysis.



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    Plasmids used in this manuscript.

    Journal: Methods in enzymology

    Article Title: Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars

    doi: 10.1016/bs.mie.2017.06.003

    Figure Lengend Snippet: Plasmids used in this manuscript.

    Article Snippet: Transform plasmid into freshly-competent BL21 (DE3) RIL cells, growing transformants on LB-agar selection media containing corresponding vector selection antibiotic (see ) and 30 μg/mL CAM. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Construct Vector Resistance Reference Addgene ID GST-PglFΔ1–130 ( Cj ) pGEX4T-2 Carb, 50 μg/mL Olivier et al., 2006 89708 His 8 -TEV-PglC ( Ng ) modified pET30b(+) Kan, 30 μμg/mL Hartley et al., 2011 89709 T7-PglD-His 6 ( Cj ) pET24a(+) Kan, 30 μg/mL Morrison et al., 2010 89710 PseB-His 6 ( Cj ) pET-24a(+) Kan, 30 μg/mL this report 89723 His 8 -TEV-PseC ( Cj ) modified pET30b(+) Kan, 30 μg/mL Hartley et al., 2011 89724 His 8 -TEV-PseH ( Cj ) modified pET30b(+) Kan, 30 μg/mL Hartley et al., 2011 89725 T7-WbpB-His 6 ( Pa ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2009 89711 T7-WbpE-His 6 ( Pa ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2009 89712 T7-WbpD-His 6 ( Pa ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2009 89713 T7-AglK-His 6 ( Mv ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2013 89714 T7-AglC-His 6 ( Mv ) pET-24a(+) Kan, 30 μg/mL Larkin et al., 2013 89726 Open in a separate window Plasmids used in this manuscript.

    Techniques: Plasmid Preparation, Modification